Metric · Anaerobic Threshold (MLSS)

MLSS. Maximal Lactate Steady State.

Maximal Lactate Steady State (MLSS) describes the highest exercise intensity, expressed as power or speed, at which blood lactate concentration remains stable over time. It represents an equilibrium between lactate production and lactate combustion: lactate is produced and utilized at the same rate, preventing progressive lactate accumulation.

In incremental exercise testing it is approximated using lactate profile curves which determine a proxy of MLSS called anaerobic threshold or LT2.

Lactate production vs. lactate combustion

la.-productionmax. aerobic la.-combustionMLSS
501002003004005006006970123455.5mmol/l/minMLSS

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What MLSS is not.

MLSS is not the same as Functional Threshold Power (FTP), nor is it simply interchangeable with Critical Power or Critical Speed. These metrics are determined using different methodologies and should not automatically be treated as equivalent.

The methodological differences are documented in Borszcz et al., 2019 for FTP, and in Caen et al., 2024 and Borszcz et al., 2024 for critical power.

MLSS and training zones.

MLSS, or anaerobic threshold, is commonly used as a reference point for defining training zones. Training intensities are often prescribed as percentages above or below threshold power or speed.

However, the widespread use of threshold-based training zones is partly historical. Incremental exercise testing became one of the most accessible methods for physiological performance assessment, and lactate-derived thresholds therefore became convenient reference points for exercise prescription. This does not necessarily mean that a fixed percentage of MLSS is the physiologically optimal way to define an individual athlete’s training zones.

MLSS is therefore an important performance benchmark, but its usage to define training zones is primarily historical and not based on physiological science.

Percentage zones while VO₂max and VLamax change

conventional zonephysiological metricMLSS
The power output at MLSS is regularly implemented to define training zones (Frontiers in Sports and Active Living, 2024), with zone limits set as fixed percentages of it (Binder et al., 2008). When VO₂max and VLamax change together, MLSS can stay at the same power, so the zones stay the same, while FatMax, VO₂max power and the intensity at 60 g/h carbohydrate combustion move.

The gold standard for measuring MLSS.

The gold-standard method for determining MLSS requires 30 min test of constant load with repeated measurement of lactate concentration to find the highest intensity at which lactate remains stable and doesn’t accumulate with time.

Because finding this intensity usually requires multiple constant-load trials on separate occasions, direct MLSS determination is highly time-consuming and impractical for routine performance testing.

For this reason, incremental lactate tests are commonly used to estimate MLSS instead. However, the endless variations and combinations of test protocols and different concepts to approximate MLSS (from fixed 4 mmol/L to DMAX and other methods) makes detection of MLSS ambiguous and unprecise.

The key distinction: a direct 30-minute constant-load protocol measures MLSS; an incremental lactate test approximates it, in most cases with unknown accuracy.

Three constant-load trials, 30 minutes each

below MLSSMLSSabove MLSS
below MLSSMLSSabove MLSS02468051015202530blood lactate, mmol/ltime, min
The gold-standard protocol: repeated constant-load bouts of about 30 minutes, each at a higher power, with lactate sampled throughout. The highest bout at which the concentration stays stable is MLSS; one step higher, lactate accumulates until the trial ends. Direct determination takes two to five such trials (Heck & Wackerhage, 2024; Frontiers in Sports and Active Living, 2024).

Determine MLSS from its physiological origin.

INSCYD determines MLSS from its physiological definition: the exercise intensity at which lactate production and lactate combustion are in equilibrium.

Rather than identifying a fixed lactate concentration or applying a threshold algorithm to the shape of an incremental lactate curve, the calculation determines where the athlete’s individual rates of lactate production and combustion reach this balance.

This makes the determination independent of the testing protocols such as step duration, workload increments or the lactate concentrations reached during individual stages.

The method has been validated against directly measured MLSS in peer-reviewed research and proven to be likely the most accurate way to determine MLSS, with superior accuracy over most if not all lactate profile methods.

Instead of approximating MLSS from a specific test protocol, INSCYD determines it from the physiological processes that create it.

The centre does not move

Lab testField testLactateNo lactateStep durationWorkload incrementBody composition / individual physiology
One physiological definition of MLSSLactate production=Lactate combustion
Everything on the outside varies from test to test; the definition in the centre does not. The calculation built on it agrees with directly measured MLSS in the published validations (Frontiers in Sports and Active Living, 2024; Podlogar et al., 2022).
0.992rINSCYD-calculated versus directly measured MLSS, mean difference 4.6 W, within the reported typical error of direct MLSS determination (Frontiers in Sports and Active Living, 2024)
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