Maximal Lactate Steady State (MLSS) describes the highest exercise intensity, expressed as power or speed, at which blood lactate concentration remains stable over time. It represents an equilibrium between lactate production and lactate combustion: lactate is produced and utilized at the same rate, preventing progressive lactate accumulation.
In incremental exercise testing it is approximated using lactate profile curves which determine a proxy of MLSS called anaerobic threshold or LT2.
From national federations and Olympic programmes to WorldTour teams and professional clubs worldwide.
MLSS is not the same as Functional Threshold Power (FTP), nor is it simply interchangeable with Critical Power or Critical Speed. These metrics are determined using different methodologies and should not automatically be treated as equivalent.
The methodological differences are documented in Borszcz et al., 2019 for FTP, and in Caen et al., 2024 and Borszcz et al., 2024 for critical power.
MLSS, or anaerobic threshold, is commonly used as a reference point for defining training zones. Training intensities are often prescribed as percentages above or below threshold power or speed.
However, the widespread use of threshold-based training zones is partly historical. Incremental exercise testing became one of the most accessible methods for physiological performance assessment, and lactate-derived thresholds therefore became convenient reference points for exercise prescription. This does not necessarily mean that a fixed percentage of MLSS is the physiologically optimal way to define an individual athlete’s training zones.
MLSS is therefore an important performance benchmark, but its usage to define training zones is primarily historical and not based on physiological science.
MLSS represents an equilibrium of the metabolism, a sustainable intensity which doesn’t lead to failure or exhaustion unless the athlete runs out of fuel in terms of carbohydrates. In contrast, racing and competing implies maxing out one’s physiological capacities.
For this reason, no competition is performed entirely and exactly at MLSS. Most events are performed close to, somewhat above, or below this intensity. Depending on the athlete and event duration, performances such as cycling time trials, 10-km running or elite-level half-marathon running may occur relatively close to MLSS, while longer events are generally performed below it.
MLSS is therefore useful not because athletes necessarily race exactly at MLSS, but because it provides a physiological reference point against which race intensity and endurance performance can be understood.
MLSS is not a physiological system, such as the aerobic or glycolytic energy system by itself. Unlike VO₂max or VLamax, it does not describe the maximum capacity of a single energy system. Instead, MLSS is an outcome of the interaction between different metabolic processes.
Two of the most important determinants are VO₂max and VLamax. VLamax strongly influences the rate at which lactate is produced in glycolysis, while aerobic capacity determines the ability to utilize lactate and produce energy oxidatively. MLSS occurs at the exercise intensity at which these opposing processes remain in equilibrium.
Consequently, changing VO₂max or VLamax changes MLSS. With other factors remaining equal, a higher VO₂max allows a higher power or speed at MLSS. Conversely, a higher VLamax increases glycolytic lactate production and can reduce the power or speed at which lactate production and combustion remain balanced.
If the goal is to understand why MLSS is where it is, or how to increase it through training, VO₂max and VLamax need to be measured.
The gold-standard method for determining MLSS requires 30 min test of constant load with repeated measurement of lactate concentration to find the highest intensity at which lactate remains stable and doesn’t accumulate with time.
Because finding this intensity usually requires multiple constant-load trials on separate occasions, direct MLSS determination is highly time-consuming and impractical for routine performance testing.
For this reason, incremental lactate tests are commonly used to estimate MLSS instead. However, the endless variations and combinations of test protocols and different concepts to approximate MLSS (from fixed 4 mmol/L to DMAX and other methods) makes detection of MLSS ambiguous and unprecise.
The key distinction: a direct 30-minute constant-load protocol measures MLSS; an incremental lactate test approximates it, in most cases with unknown accuracy.
INSCYD determines MLSS from its physiological definition: the exercise intensity at which lactate production and lactate combustion are in equilibrium.
Rather than identifying a fixed lactate concentration or applying a threshold algorithm to the shape of an incremental lactate curve, the calculation determines where the athlete’s individual rates of lactate production and combustion reach this balance.
This makes the determination independent of the testing protocols such as step duration, workload increments or the lactate concentrations reached during individual stages.
The method has been validated against directly measured MLSS in peer-reviewed research and proven to be likely the most accurate way to determine MLSS, with superior accuracy over most if not all lactate profile methods.
Instead of approximating MLSS from a specific test protocol, INSCYD determines it from the physiological processes that create it.
The size of the aerobic engine, and the capacity to utilize lactate oxidatively.
Read more VLamaxMaximum glycolytic power: how fast lactate is produced in the first place.
Read more Lactate Threshold 1 (LT1)The first rise above resting lactate, and the lower boundary of the same profile.
Read moreVO₂max, VLamax, FatMax, thresholds, fuel use and training zones, from a single test in the lab, in the field or fully remote.